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| OEM | OEM Services Provided |
| Feature | Good stability,Easy to Use |
| Classification | Dyes and Pigments |
| Advantage | Professional, Fast Delivery, Customizable | Samples | Apply for free，Contact us now. |
| Product Name: | Sulforhodamine B |
| Synonyms: | KitonRedSC.I.14895;SULFORHODAMINE B MONOSODIUM SALT;SULFORHODAMINE B;PHLOXINE RHODAMINE;XYLENE RED;XYLENE RED B;Xanthylium, 3,6-bis(diethylamino)-9-(2,4-disulfophenyl)-, inner salt, sodium salt;sulforhodamine b sodium salt |
| CAS: | 3520-42-1 |
| MF: | C27H31N2NaO7S2 |
| MW: | 582.66 |
| EINECS: | 222-529-8 |
| Product Categories: | acid dyes;Stains, Dyes and Indicators;Cell Viability and Proliferation;Reagents and Supplements;S;Stains and Dyes;Stains&Dyes, A to;Organics;Analytical Chemistry;Food Colors by Food Sanitation Law in Japan |
| Mol File: | 3520-42-1.mol |
| density | 1.472[at 20℃] |
| storage temp. | room temp |
| solubility | methanol: 1 mg/mL |
| Colour Index | 45100 |
| form | powder |
| color | Brown to Very Dark Purple |
| biological source | synthetic (organic) |
| Water Solubility | 95.3g/L at 20℃ |
| λmax | 554 nm |
| ε(extinction coefficient) | ≥85000 at 554-560nm in 0.1 M NaOH in methanol at 0.005g/L |
| BRN | 3886008 |
| Cosmetics Ingredients Functions | COLORANT HAIR DYEING |
| InChIKey | XCRMYPBRGZRYEU-UHFFFAOYSA-N |
| SMILES | S(C1C=C(S(O)(=O)=O)C=CC=1C1C2=CC=C(N(CC)CC)C=C2[O+]=C2C=C(N(CC)CC)C=CC=12)([O-])(=O)=O.[NaH] |
| LogP | -2.2 at 20℃ |
| EPA Substance Registry System | C.I. Acid Red 52 (3520-42-1) |
| Hazard Codes | Xi |
| Risk Statements | 36/37/38 |
| Safety Statements | 22-24/25-26-36/37/39 |
| WGK Germany | 2 |
| RTECS | BP6750000 |
| F | 8 |
| TSCA | Yes |
| HS Code | 32129000 |
| Provider | Language |
|---|---|
| SigmaAldrich | English |
| ALFA | English |
| Description | Sulforhodamine B is a membrane-impermeant polar tracer. | |||||||||||||||||||||||||||||||||
| This substance is a deep red powder. It is easily soluble in water, forming a bluish-red solution with yellow fluorescence. When sodium hydroxide solution is added, it also appears bluish-red. Additionally, it is soluble in ethanol. In concentrated sulfuric acid, it turns orange-yellow and red after dilution. | ||||||||||||||||||||||||||||||||||
| Uses | Sulforhodamine B Sodium Salt is a rhodamine dye widely used in nonpermanent tattoos. Studies show that Sulforhodamine B can be used as a potential new therapeutic agent for the treatment of the acute respiratory distress syndrome. Dyes and metabolites, Environmental Testing | |||||||||||||||||||||||||||||||||
Sulforhodamine B sodium salt has been used:
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| Application | Sulforhodamine B (SRB) is known for use in SRB assay for the determination of in vitro cytotoxicity through staining and the observation of live cells, proliferation and drug screening. It has been used in the SRB assay to evaluate the effect of mouthwashes on protein content in primary cultures of human fibroblasts. It is also suitable as a microscopic tracer for the assessment of blood-brain barrier integrity by post mortem inspection of frozen sections. SRB is useful as a caries detector dye with subsequent inspection of histological material. | |||||||||||||||||||||||||||||||||
| ChEBI: Sulforhodamine B is an organic sodium salt having 4-[3,6-bis(diethylamino)-2,7-dimethylxanthenium-9-yl]benzene-1,3-disulfonate as the counterion. | ||||||||||||||||||||||||||||||||||
| Preparation | 4-Formylbenzene-1,3-disulfonic acid and 3-(Diethylamino)phenol condensation, the products with sulfuric acid dehydration, and ferric chloride oxidation, and finally into sodium salt. | |||||||||||||||||||||||||||||||||
| Sulforhodamine B (SRB) is a bright-pink aminoxanthene dye. The sulforhodamine B (SRB) assay is extensively used for in vitro cytotoxicity screening. SRB binds to the protein constituents of the cells in a stoichiometric manner. Thus, the amount of dye extracted from stained cells is directly proportional to the cell mass. SRB can also be used for cell density determination. | ||||||||||||||||||||||||||||||||||
| Flammability and Explosibility | Not classified | |||||||||||||||||||||||||||||||||
| Sulforhodamine B sodium salt is useful to measure cell biomass and also for cytotoxicity screening. | ||||||||||||||||||||||||||||||||||
| Properties and Applications | bright blue light pink. Dark red light brown powder. Soluble in water, in blue light red, with fluorescence, also soluble in alcohol. The strong sulfuric acid dyes is orange, diluted for red, with sodium hydroxide solution is blue light red. Mainly used for wool, silk and polyamide fiber dyeing, also can be used for wool and a variety of fabric dyeing, still can be in wool and silk fabric printing directly, also can used for leather dyeing.
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| Raw materials | Sulfuric acid-->Toluene-->Sodium carbonate-->Potassium permanganate-->FUMING SULFURIC ACID-->Potassium dichromate-->4-Hydroxybenzenesulfonic acid-->3-Diethylaminophenol-->Manganese sulfate-->3-Diethylaminophenol-->Benzaldehyde-2,4-disulfonic acid |
Declaration: The products displayed on this website are intended exclusively for industrial applications or scientific research. They are not intended for medical, pharmaceutical, or food use. In accordance with applicable laws and regulations, purchasing organizations must hold valid qualifications and approvals.
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